When
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- Dates offered: March 9-10, 2023
- Maximum course enrollment: 8-10
- Length of time: 2 days - in-person
Day 1: Lecture & Demonstrations & Lab Exercise
Day 2: Laboratory Exercise
The Seed Pathogen Testing workshop for 2023 is for two days at the PPQ S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), Laurel, MD. It will comprise of a lecture overview of seedborne pathology and the principles of extraction of nucleic acid from seeds for pathogen detection. There will a hands-on laboratory exercise on seed nucleic acid extraction and RT-PCR detection of a seedborne virus performed by each participant. Laboratory exercise results will be analyzed, and experience shared. Seed grinding tools and equipment, and robotic extraction of nucleic acid from seeds will be demonstrated. Nucleic acid extraction will be magnetic bead based.
*Note: ALL times listed for NPDN events shall be listed as Eastern Time.
