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Quick & Useful Tips

🔔 After 24 years of continuous USDA NIFA cooperative agreement support, delays in the next funding opportunity have required NPDN to strategically scale back operations. Remaining funds under a no-cost extension are being used to preserve critical infrastructure and sustain essential national functions. As of September 1, 2026, network activities will be paused or operate at reduced capacity, with full operations expected to resume when the next cooperative agreement and associated funding are in place.

  • Utilization of locked nucleic acids in PCR primers and probes to enhance specificity
  • Buying probes for real-time PCR? Check if you need an MGB probe!
  • Neopestalotiopsis on strawberries
  • A Checklist for Triage of Plant Diagnostic Samples
  • P&V Tips: In the Zone: Identifying Zonate Leaf Spot
  • Sample adequacy controls (SAC) for molecular detection methods
  • Best Practices for Ordering and Handling qPCR Probes
  • Tips for Identifying Fusarium - Part 2: Sequence-based Identification
  • Tips for identifying Anthracnose fruit rot in strawberries
  • Tips for the purification of fungi and oomycetes
  • Tips for Identifying Fusarium - Part 1: Morphological Identification
  • Common Needle Diseases and Disorders of Spruce and Pine
  • Tips for identifying Rhizoctonia
  • Tissue Homogenization for Nucleic Acid Extraction Tips
  • Mushroom Identification Tips
  • Tips for coping with PCR inhibitors
  • Older Diagnostics Tips (before 2020)

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NPDN supports plant health and biosecurity in U.S. agricultural and natural ecosystems by providing expert diagnostic capacity, communication, coordination, and quality pest and disease diagnostic information.

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